Although there is increasing evidence about the role of vitamin D on muscle function, its relationship with grip strength is still a controversial issue. In this cross-sectional clinical study, it was planned to evaluate the relationship between vitamin D and grip strength in premenopausal Turkish women. A total of 127 women with age range of 40-50 years (mean age: 44,7±4,3), premenopausal, sedentary, were included in this cross-sectional descriptive study. The mean BMI was 30.2 ± 5.3 kg/m2. Dominant and nondominant grip strength was measured by digital hand dynamometer. The mean dominant grip strength was 24.2 ± 5.9 kg and the mean nondominant grip strength was 22.5 ± 5.7 kg. Mean serum 25-hydroxyvitamin D (25OHD) concentrations were 16.4 ± 9.7 ng / ml. Participants were divided into 3 groups as vitamin D deficiency (70.9%), insufficiency (18.1%), sufficiency (11.0%). No statistically significant difference was found between the groups in terms of age and body mass index (BMI), dominant and nondominant grip strength (p> 0.05). Furthermore, no significant relationship was found between serum 25OHD concentration and dominant and nondominant grip strength (p> 0.05). In addition, BMI was not associated with dominant and nondominant grip strength (p> 0.05). These results of this study provide evidence that vitamin d is not effective on grip strength at least in premenopausal Turkish women.Keywords: Cross-sectional study, grip strength, serum 25OHD, vitamin D deficiency.