Symptomatic anastomotic leakage observed after colorectal surgery is one of the major complications. One of the factors affecting anastomosis healing is the suture materials used. This study aims to investigate the effects of the suture material Polydioxanone (PDS®, Ethicon), which is late absorbable and has a monofilament structure, and the suture material Polyglactin 910 (Vicryl®, Ethicon), which is absorbable and has a multifilament structure, on the healing of colonic anastomosis and to compare the traditional forms of these sutures with their antibacterial effective triclosan impregnated forms (PDS Plus®, Ethicon and Vicryl Plus®, Ethicon). The rats were divided into four equal groups consisting of 10 subjects each: Group I: Vicryl, Group II: Vicryl Plus®, Group III: PDS, Group IV: PDS Plus®. The presence of wound infection, whether the integrity of the abdominal wall was maintained, intra-abdominal adhesion scoring, the presence of intra-abdominal abscess, and whether the macroscopic integrity of anastomosis was maintained were evaluated. According to the results of this experimental study, while the highest ABP and hydroxyproline levels were observed in the PDS Plus group, the lowest values were observed in the Vicryl group. However, the difference was not statistically significant. Slowly absorbable and monofilament PDS suture material causes less tissue reaction and inflammatory response compared to Vicryl suture material that is absorbable in the colonic anastomosis line and multifilament.Keywords: Polydioxanone, Polyglactin 910, Triclosan, Colonic anastomosis, hydroxyproline.