Objective: There are several comparison studies to explain the specific properties of oral tissue derived mesenchymal stem cells. However, apical papilla stem cells from supernumerary tooth (ST-APSCs) have not been characterized for their MSC properties yet.
Methods: In the current study, ST-APSCs were isolated and characterized from a nonsyndromic male patient to evaluate the MSC characteristics. DPSCs and ST-APSCs were isolated and characterized for mesenchymal surface markers. Cells were differentiated into osteo-, chondro- and adipogenic cell types.
Results: Both cell types expressed MSC surface markers. When DPSCs and ST-APSCs were cultured in differentiation media promoting transformation to osteo-, chondro- and adipo-genic lineages, both showed calcium mineralization, chondrogenic mass formation and lipid accumulation. However, DPSCs derived from wisdom tooth demonstrated more differentiation potential to osteo- and chondro-genic cell types compared to ST-APSCs.
Conclusion: Overall, ST-APSCs were characterized by their MSC properties and able to differentiate into three cell lineages. However, they are less potent for osteo- and chondro-genic cell lineage specification compared to DPSCs derived from wisdom tooth.